Multiple color single molecule TIRF imaging and tracking of MAPs and motors.

Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).

Resolving single fluorescent molecules in the presence of high fluorophore concentrations remains a challenge in single-molecule biophysics that limits our understanding of weak molecular interactions. Total internal reflection fluorescence (TIRF) imaging, the workhorse of single-molecule fluorescence microscopy, enables experiments at concentrations up to about 100 nM, but many biological inte...

Chapter 27 – Studying Plus-End Tracking at Single Molecule Resolution Using TIRF Microscopy

Versatile single-molecule multi-color excitation and detection fluorescence setup for studying biomolecular dynamics.

Single-molecule fluorescence imaging is at the forefront of tools applied to study biomolecular dynamics both in vitro and in vivo. The ability of the single-molecule fluorescence microscope to conduct simultaneous multi-color excitation and detection is a key experimental feature that is under continuous development. In this paper, we describe in detail the design and the construction of a sop...

Steady-State Performance Characteristics of Photovoltaic System Coupled with a Centrifugal Water Pump

In this article our objective is to obtain steady state characteristics of shunt, series, and separately excited DC motors coupled with a centrifugal water pump supplied through a step-up, step-down, and cuk converter from photovoltaic generator. We assume that there are no storage batteries and, of course, the system must operate on maximum utilization i.e. maximum water must be pumped. It is ...

Steady-State Performance Characteristics of Photovoltaic System Coupled with a Centrifugal Water Pump

In this article our objective is to obtain steady state characteristics of shunt, series, and separately excited DC motors coupled with a centrifugal water pump supplied through a step-up, step-down, and cuk converter from photovoltaic generator. We assume that there are no storage batteries and, of course, the system must operate on maximum utilization i.e. maximum water must be pumped. It is ...